SAGE Project Summary Form

Title: yeast cells arrested in S phase with hydroxyurea

SAGE Software Project Files

MS Access File

Date of Library Completion: August 1996

Principal SAGE Master: Victor Velculescu

Number of Tags: 20,773

Number of Sequence Files: 791 Tags/Sequence File: 26.2

Number of Duplicate DiTags: 723 Percent: 6.9%

Number of Linker Tags: 368 Percent Linker Tags: 1.7%

Reference:

1) Velculescu V.E., Zhang L., Zhou W., Vogelstein J., Basrai M.A., Bassett D. Jr., Hieter P., Vogelstein B., Kinzler, K.W. Characterization of the yeast transcriptome. Cell, 88: 243-251, 1997.

2) Velculescu VE, Zhang L, Vogelstein B, and Kinzler KW (1995). Serial Analysis Of Gene Expression. Science 270, 484-487.

Detailed Description:

We have analyzed the set of genes expressed from the yeast genome, called the transcriptome, using serial analysis of gene expression (SAGE). In order to maximize representation of genes involved in normal growth and cell-cycle progression SAGE libraries were generated from yeast cells in three states: log phase, S phase arrested and G2/M phase arrested. Analysis of 60,633 transcripts revealed 4,665 genes, with expression levels ranging from 0.3 to over 200 transcripts per cell. Of these genes, 1,981 had known functions, while 2,684 were previously uncharacterized. Integration of positional information with gene expression data allowed the generation of chromosomal expression maps, identifying physical regions of high and low transcriptional activity, and identified genes that had not been predicted by sequence information alone. These studies provide insight into global patterns of gene expression in yeast and demonstrate the feasibility of genome-wide expression studies in eukaryotes.

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