SAGE Project Summary Form
Title: yeast cells arrested in S phase with
Software Project Files
Date of Library Completion: August 1996
Principal SAGE Master: Victor Velculescu
Number of Tags: 21,360
Number of Sequence Files: 839 Tags/Sequence
Number of Duplicate DiTags: 1264 Percent: 11.2%
Number of Linker Tags: 1326 Percent Linker Tags:
1) Velculescu V.E., Zhang L., Zhou W., Vogelstein
J., Basrai M.A., Bassett D. Jr., Hieter P., Vogelstein B.,
Kinzler, K.W. Characterization of the yeast transcriptome.
Cell, 88: 243-251, 1997.
2) Velculescu VE, Zhang L, Vogelstein B, and
Kinzler KW (1995). Serial
Analysis Of Gene Expression. Science 270, 484-487.
We have analyzed the set of genes expressed
from the yeast genome, called the transcriptome, using serial
analysis of gene expression (SAGE). In order to maximize representation
of genes involved in normal growth and cell-cycle progression
SAGE libraries were generated from yeast cells in three states:
log phase, S phase arrested and G2/M phase arrested. Analysis
of 60,633 transcripts revealed 4,665 genes, with expression
levels ranging from 0.3 to over 200 transcripts per cell.
Of these genes, 1,981 had known functions, while 2,684 were
previously uncharacterized. Integration of positional information
with gene expression data allowed the generation of chromosomal
expression maps, identifying physical regions of high and
low transcriptional activity, and identified genes that had
not been predicted by sequence information alone. These studies
provide insight into global patterns of gene expression in
yeast and demonstrate the feasibility of genome-wide expression
studies in eukaryotes.