SAGE Project Summary Form

Title: HCT116

SAGE Software Project Files

MS Access File

Date of Library Completion: August 1995

Principal SAGE Master: Lin Zhang

Number of Tags: 59,572

Number of Sequence Files: 2,901 Tags/Sequence File: 20.5

Number of Duplicate DiTags: 1,855 Percent: 6.22%

Number of Linker Tags: 3,450 Percent Linker Tags: 5.80%

Reference:

1) Zhang L, Zhou W, Velculescu VE, Kern SE, Hruban RH, Hamilton SR, Vogelstein B, Kinzler KW (1997) Science 276(5316):1268-72. 05/23/97

2) Velculescu VE, Zhang L, Vogelstein B, and Kinzler KW (1995). Serial Analysis Of Gene Expression. Science 270, 484-487.

Detailed Description:

As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined in gastrointestinal tumors and normal colon tissues. Hct116 is a mismatch repair deficient colon cancer cell line. PolyA RNA was isolated from subconfluent Hct116 cells and SAGE was performed. More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insight into the extent of expression differences underlying malignancy and reveal genes that may prove useful as diagnostic or prognostic markers.

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