SAGE Project Summary Form

Title: Pancreatic Primary Tumor 96-6252

SAGE Software Project Files

MS Access File

Date of Library Completion: May 1996

Principal SAGE Master: Wei Zhou

Number of Tags: 34, 416

Number of Sequence Files: 1,210 Tags/Sequence File: 28.4

Number of Duplicate DiTags: 1034 Percent Duplicate (2X Ditag/Total): 6.00%

Number of Linker Tags: 3,121 Percent Linker Tags: 9.06%

Reference:

1) Zhang L, Zhou W, Velculescu VE, Kern SE, Hruban RH, Hamilton SR, Vogelstein B, Kinzler KW (1997) Science 276(5316):1268-72. 05/23/97

2) Velculescu VE, Zhang L, Vogelstein B, and Kinzler KW (1995). Serial Analysis Of Gene Expression. Science 270, 484-487.

Detailed Description:

As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined in gastrointestinal tumors and normal colon tissues. A frozen tumor sample was obtained from a colon cancer patient. Histopathology confirmed that the tumor sample contained at least 70% of tumor cells. PolyA RNA was isolated and SAGE was performed. More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insight into the extent of expression differences underlying malignancy and reveal genes that may prove useful as diagnostic or prognostic markers.

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