Ferredoxin reductase affects p53-dependent, 5-fluorouracil-induced apoptosis in colorectal cancer cells

 

Paul M. Hwang, Fred Bunz, Jian Yu, Carlo Rago, Timothy A. Chan, Michael P. Murphy, Geoffry F. Kelso, Robin A. J. Smith, Kenneth W. Kinzler KW, Bert Vogelstein.

Nature Medicine. 2001 October 7, pp 1111-1117 (2001).

Supplemental Material

Complete list of genes induced or repressed following 5-FU treatment (P-False < 0.05).  WT, number of SAGE tags in p53+/+ HCT116 treated with 5-FU; KO, number of SAGE tags in p53-/- HCT116; WT/KO, fold induction of SAGE tags.  Description indicates SAGE tag match to Unigene ID, with blank space indicating no reliable match.

Tag_Sequence WT KO WT/KO Unigene ID Description
GAGGCCAACA 13 0 13.00 50649 quinone oxidoreductase homolog
GCAACGGGCC 15 0 15.00 8679 cytosolic acyl coenzyme A thioester hydrolase
GGGGCAAAAA 12 0 12.00    
GTGCTCATTC 24 0 24.00 116577 prostate differentiation factor
GTGCTCATTC 24 0 24.00 25945 ESTs
TGGACCCCCC 13 0 13.00 173501 Homo sapiens mRNA for FLJ00008 protein, partial cds
TGGGTGGGGG 18 1 18.00 25197 STIP1 homology and U-Box containing protein 1
GCGGCAGCGG 17 1 17.00 171374 smg GDS-ASSOCIATED PROTEIN
GTGGTGGGCA 17 1 17.00    
TGCCCAGGAT 16 1 16.00 91448 MKP-1 like protein tyrosine phosphatase
GCGGCGGCTC 30 2 15.00 4437 ribosomal protein L28
AGAGAAATTT 14 1 14.00 77028 protein translocation complex beta
AGGGGCGCAG 13 1 13.00 97616 SH3-domain GRB2-like 1
GGGAGGAGGG 13 1 13.00 296370 ESTs
CTGGAAATAA 22 2 11.00 69745 ferredoxin reductase
CTGGAAATAA 22 2 11.00 75576 plasminogen
ACCAAATTAA 19 2 9.50 51233 tumor necrosis factor receptor superfamily, member 10b
ACCCGCCGGG 19 2 9.50    
TGGCGTGGCC 28 3 9.33 8854 Human transcription unit PVT gene, exons I-III
GCGGCTTTCC 17 2 8.50 204354 ras homolog gene family, member B
GCGGCTTTCC 17 2 8.50 278431 SCO (cytochrome oxidase deficient, yeast) homolog 2
GGGTAGCTGG 17 2 8.50 172182 poly(A)-binding protein, cytoplasmic 1
AACCCAGGAG 16 2 8.00    
CTCCACAAAT 24 3 8.00 278426 PDGFA associated protein 1
GGTGCAGAGC 38 5 7.60 183353 ESTs, Weakly similar to
GGTGCAGAGC 38 5 7.60 539 ribosomal protein S29
CAAATAAAAA 20 3 6.67 1116 lymphotoxin beta receptor (TNFR superfamily, member 3
CAAATAAAAA 20 3 6.67 21198 translocase of outer mitochondrial membrane 70 (yeast) homolog A
CAAATAAAAA 20 3 6.67 74034 caveolin 1, caveolae protein, 22kD
GGCAACGTGG 20 3 6.67 80162 Huntingtin interacting protein K
GGCTGGGTTT 20 3 6.67 74870 H2.0 (Drosophila)-like homeo box 1
TGTCCTGGTT 73 11 6.64 179665 cyclin-dependent kinase inhibitor 1A (p21, Cip1)
GGGCGGGGGC 32 5 6.40 165904 ESTs
GGGCGGGGGC 32 5 6.40 300790 ESTs, Highly similar to
GGGCGGGGGC 32 5 6.40 31549 KIAA1501 protein
GGGCGGGGGC 32 5 6.40 99890 polymerase (DNA directed), delta 1, catalytic subunit (125kD)
GTGTTGGGGG 25 4 6.25 55016 hypothetical protein FLJ21935
GGCCCGAGTT 23 4 5.75 179606 nuclear RNA helicase, DECD variant of DEAD box family
GCGAAACCCC 88 18 4.89    
GGCCGCGTTC 47 10 4.70 5174 ribosomal protein S17
GGGAAAAAAA 26 6 4.33 279841 hypothetical protein FLJ10335
GCAAGCCAAC 47 11 4.27    
GTGGCGGGCA 25 6 4.17    
GTGGCGGGCG 83 21 3.95    
GGCTTTAGGG 38 10 3.80    
GCCCCCCCGT 34 9 3.78 228309 EST
GCCCCCCCGT 34 9 3.78 85573 Homo sapiens mRNA; cDNA DKFZp566N034 (from clone DKFZp566N034); partial cds
GGCCCTGAGC 119 32 3.72 71618 polymerase (RNA) II (DNA directed) polypeptide L (7.6kD)
GCGAAACCCT 26 7 3.71    
GGGATGGCAG 37 10 3.70 159637 valyl-tRNA synthetase 2
AGCCCGCCGC 33 9 3.67 154036 tumor suppressing subtransferable candidate 3
GGGACGGGTG 29 8 3.63    
GGGGTAAGAA 38 11 3.45 80423 prostatic binding protein
GTGGCAGGCG 43 13 3.31    
GGGGCAGGGC 49 16 3.06 119140 eukaryotic translation initiation factor 5A
GGGCCCAGGA 33 11 3.00 118983 Homo sapiens cDNA FLJ12150 fis, clone MAMMA1000422
GATCCCAACT 47 16 2.94 118786 metallothionein 2A
GAGAGCTCCC 38 13 2.92 mito Tag matches mitochondrial sequence
CGCCGCGGTG 34 12 2.83 4835 eukaryotic translation initiation factor 3, subunit 8 (110kD)
CCCCCTGGAT 78 28 2.79 275243 S100 calcium-binding protein A6 (calcyclin)
CCCGTCCGGA 307 113 2.72 180842 ribosomal protein L13
AATCTGCGCC 35 13 2.69 833 interferon-stimulated protein, 15 kDa
GCCAGCCCAG 51 20 2.55 228059 KRAB-associated protein 1
GGGGGTCACC 94 37 2.54 80986 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1
GGCAGAGGAC 104 44 2.36 118638 non-metastatic cells 1, protein (NM23A) expressed in
GGGCCTGTGC 47 20 2.35 146517 ESTs
GGGCCTGTGC 47 20 2.35 85838 solute carrier family 16 (monocarboxylic acid transporters), member 3
GTGAAACCCC 309 136 2.27    
AGCACCTCCA 122 54 2.26 75309 eukaryotic translation elongation factor 2
GCAAAACCCC 51 23 2.22    
GGGCTGGGGT 422 192 2.20 183698 ribosomal protein L29
GCTGTTGCGC 125 57 2.19 8102 ribosomal protein S20
GCAGCCATCC 197 90 2.19 4437 ribosomal protein L28
GGGGTCAGGG 54 25 2.16 133481 Homo sapiens mRNA; cDNA DKFZp564O0862 (from clone DKFZp564O0862)
GGGGTCAGGG 54 25 2.16 75658 phosphorylase, glycogen; brain
GGGGTCAGGG 54 25 2.16 75879 ribosomal protein L19
TTTAACGGCC 80 38 2.11 mito Tag matches mitochondrial sequence
GGGCCCCAAA 62 30 2.07 256301 ESTs
CGCCGCCGGC 314 153 2.05 182825 ribosomal protein L35
GGGAAGCAGA 67 33 2.03    
AGGTGGCAAG 91 45 2.02 mito Tag matches mitochondrial sequence
AACGCGGCCA 123 62 1.98 73798 macrophage migration inhibitory factor (glycosylation-inhibiting factor)
GGCTGGGGGC 187 97 1.93 75721 profilin 1
GGACCACTGA 72 38 1.89 119598 ribosomal protein L3
GCCGAGGAAG 136 72 1.89 82148 hypothetical protein
AGGAAAGCTG 226 124 1.82 76437 ribosomal protein L36
GGAAAAAAAA 143 79 1.81 177530 ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
GTGAAACCCT 126 72 1.75    
GCGCAGAGGT 224 131 1.71 108124 ribosomal protein L41
TGCACGTTTT 304 180 1.69 169793 ribosomal protein L32
GCGACGAGGC 125 75 1.67 2017 ribosomal protein L38
GAGGGAGTTT 473 293 1.61 76064 ribosomal protein L27a
GAAAAAAAAA 227 146 1.55    
GGATTTGGCC 635 422 1.50 119500 ribosomal protein, large P2
CCCATCGTCC 270 196 1.38 mito Tag matches mitochondrial sequence
TCCCCGTACA 1131 864 1.31    
CTGGGTTAAT 273 403 0.68 126701 ribosomal protein S19
TCCCTATTAA 658 1010 0.65    
TCAGATCTTT 88 137 0.64 75344 ribosomal protein S4, X-linked
GCATTTAAAT 68 114 0.60 261802 eukaryotic translation elongation factor 1 beta 1
GCATTTAAAT 68 114 0.60 275959 eukaryotic translation elongation factor 1 beta 2
TAAAAAAAAA 147 255 0.58 3491 ribosomal protein S14
TGAGGGAATA 59 105 0.56 83848 triosephosphate isomerase 1
TAGGTTGTCT 82 151 0.54 119252 tumor protein, translationally-controlled 1
TAGGTTGTCT 82 151 0.54 279860 hypothetical protein FLJ20030
CTAAGACTTC 59 109 0.54    
CACTTGCCCT 58 112 0.52 14779 acetyl-CoA synthetase
CACTTGCCCT 58 112 0.52 15977 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22)
CTCAACATCT 37 75 0.49 73742 ribosomal protein, large, P0
GCATAATAGG 109 223 0.49 184108 ribosomal protein L21 (gene or pseudogene)
AACTAAAAAA 102 210 0.49 3297 ribosomal protein S27a
AACTAAAAAA 102 210 0.49 55921 glutamyl-prolyl-tRNA synthetase
GGCTTTACCC 30 65 0.46 119140 eukaryotic translation initiation factor 5A
GGCTTTACCC 30 65 0.46 305650 EST
TAAATAATTT 32 78 0.41 1197 heat shock 10kD protein 1 (chaperonin 10)
ATAATTCTTT 105 262 0.40 539 ribosomal protein S29
TGATAATTCA 28 70 0.40 177486 amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease)
TGTGCTAAAT 17 43 0.40 250895 ribosomal protein L34
TTCAATAAAA 166 442 0.38 177592 ribosomal protein, large, P1
AACTAACAAA 38 105 0.36 3297 ribosomal protein S27a
AATAGGTCCA 38 105 0.36 113029 ribosomal protein S25
TTTTCTGAAA 12 36 0.33 76136 thioredoxin
ATTATTTTTC 31 97 0.32 153 ribosomal protein L7
AATATGTGGG 14 44 0.32 74649 cytochrome c oxidase subunit VIc
GTAAGTGTAC 143 459 0.31    
TGGCTAGTGT 12 39 0.31 118065 proteasome (prosome, macropain) subunit, beta type, 7
TAATTCTTCT 9 30 0.30 1708 chaperonin containing TCP1, subunit 3 (gamma)
TACCATCAAT 110 375 0.29 169476 glyceraldehyde-3-phosphate dehydrogenase
TTACCTCCTT 12 41 0.29 3343 phosphoglycerate dehydrogenase
CATTTCATAA 7 26 0.27 73851 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
GTGATGGTGT 7 26 0.27 197345 thyroid autoantigen 70kD (Ku antigen)
TTCATACACC 24 91 0.26 mito Tag matches mitochondrial sequence
ATTCTCCAGT 38 147 0.26 234518 ribosomal protein L23
CTGTTGGTGA 20 78 0.26 3463 ribosomal protein S23
CTGCTATACG 12 50 0.24 132243 aminopeptidase puromycin sensitive
CTGCTATACG 12 50 0.24 180946 ribosomal protein L5
TATCTGTCTA 6 25 0.24 145279 SET translocation (myeloid leukemia-associated)
TCTGTACACC 6 25 0.24 182740 ribosomal protein S11
TACAAGAGGA 9 38 0.24 174131 ribosomal protein L6
CAATAAATGT 38 171 0.22 179779 ribosomal protein L37
TCTGTTTATC 6 27 0.22 180394 signal recognition particle 14kD (homologous Alu RNA-binding protein)
TAATAAAGGT 24 111 0.22 151604 ribosomal protein S8
TCGTCTTTAT 13 62 0.21 75538 ribosomal protein S7
TCATCATCAG 4 20 0.20 177592 ribosomal protein, large, P1
TCATCATCAG 4 20 0.20 301807 arylsulfatase D
TACTAATAAA 6 31 0.19 11565 Human DNA sequence from clone RP11-353C18 on chromosome 20 Contains ESTs, STSs, GSSs and CpG islands. Contains the NIFS gene for cysteine desulfurase, two genes for novel proteins and the gene for the splicing factor CC1.3 with a second isoform (CC1.4)
AAGAAGATAG 8 42 0.19 184776 ribosomal protein L23a
TTTCAGAGAG 4 21 0.19 75975 signal recognition particle 9kD
CCTATTTACT 9 52 0.17 113205 cytochrome c oxidase subunit IV
TACCAGTGTA 3 19 0.16 79037 heat shock 60kD protein 1 (chaperonin)
TTACCATATC 7 45 0.16 300141 ribosomal protein L39
ATTGTTTATG 6 39 0.15 181163 high-mobility group (nonhistone chromosomal) protein 17
TGTACCTGTA 23 158 0.15 278242 tubulin, alpha, ubiquitous
TCCTCAAGAT 5 36 0.14 118757 enhancer of rudimentary (Drosophila) homolog
CAATGTGTTA 3 22 0.14 74823 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 (7.5kD, MWFE)
CCAAACGTGT 3 22 0.14 181307 H3 histone, family 3A
AACTAATACT 6 46 0.13 111334 ferritin, light polypeptide
TCCTTGCTTC 2 16 0.13 94491 hypothetical protein FLJ20297
TTATAAAAGA 2 17 0.12    
ACTGGTACGT 2 18 0.11 155751 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2
AAAATACTAG 2 19 0.11 40499 dickkopf (Xenopus laevis) homolog 1
CATTTGTAAT 2 19 0.11    
CTGTACAGAC 3 29 0.10 251653 tubulin, beta, 2
TCTTAATGAA 2 22 0.09 173912 eukaryotic translation initiation factor 4A, isoform 2
TTCATTATAA 3 34 0.09 250655 prothymosin, alpha (gene sequence 28)
GCTGTTCATT 1 13 0.08 288986 survival of motor neuron 1, telomeric
GCTGTTCATT 1 13 0.08 77306 survival of motor neuron 2, centromeric
TAACTGTCTT 1 13 0.08 238205 hypothetical protein PRO2013
TCCTGTAAAG 1 13 0.08 74034 caveolin 1, caveolae protein, 22kD
TGTATAAAAA 1 13 0.08 82689 tumor rejection antigen (gp96) 1
AAAACATTAT 1 16 0.06 80917 adaptor-related protein complex 3, sigma 1 subunit
CTTAAGGATT 1 16 0.06 165998 DKFZP564M2423 protein
CATTTATCAT 0 20 0.00 227777 protein tyrosine phosphatase type IVA, member 1
CTTCTGTGTA 0 11 0.00 75305 aryl hydrocarbon receptor-interacting protein
CTTCTGTGTA 0 11 0.00 78829 ubiquitin specific protease 10
TACTAGTCCC 0 13 0.00 289088 heat shock 90kD protein 1, alpha
TAGTTGTAGG 0 12 0.00 5324 hypothetical protein
TATCACTCTG 0 11 0.00 278362 male-enhanced antigen
TGTAATCTTA 0 15 0.00    
TTGACACTTT 0 21 0.00 26136 ESTs

 

top

Copyright © 2003 Sagenet. All Rights Reserved.
Site design Academic Web Pages