|
Table 2. Previously characterized and
novel Tumor Endothelial Markers (TEMs).
The top 46 tags with
the highest tumor EC (T-EC's) to normal EC (N-EC's)
tag ratios are listed in descending order. To calculate
tag ratios, a value of 0.5 was assigned in cases where
zero tags were observed. The SAGE libraries are the
same as those listed in Table 1. Tag numbers for each
group were normalized to 100,000 transcripts. A 'Description'
of the gene product corresponding to each tag is given,
followed by alternative names in parenthesis. †;
multiple tags for this gene are due to alternative polyadenylation
sites.
|
no.
|
Tag Sequence
|
N-EC's
|
T-EC's
|
HUVEC
|
HMVEC
|
Cell Lines
|
Description
|
|
1
|
GGGGCTGCCCA
|
0
|
28
|
0
|
2
|
0
|
TEM1
|
|
2
|
GATCTCCGTGT
|
0
|
25
|
0
|
0
|
0
|
TEM2
|
|
3
|
CATTTTTATCT
|
0
|
23
|
0
|
0
|
0
|
TEM3
|
|
4
|
CTTTCTTTGAG
|
0
|
22
|
6
|
20
|
1
|
regulated
in glioma-like 7-1 (Dkk-3/ REIC)
|
|
5
|
TATTAACTCTC
|
0
|
21
|
1
|
3
|
1
|
TEM4
|
|
6
|
CAGGAGACCCC
|
0
|
16
|
2
|
0
|
0
|
MMP-11 (stromelysin
3)
|
|
7
|
GGAAATGTCAA
|
1
|
31
|
53
|
22
|
1
|
MMP-2 (gelatinase
A, 72kD type IV collagenease)
|
|
8
|
CCTGGTTCAGT
|
0
|
15
|
0
|
0
|
0
|
ESTs
|
|
9
|
TTTTTAAGAAC
|
0
|
14
|
1
|
4
|
0
|
TEM5
|
|
10
|
TTTGGTTTTCC
|
5
|
139
|
0
|
16
|
0
|
collagen,
type I, alpha 2, transcript A†
|
|
11
|
ATTTTGTATGA
|
0
|
13
|
4
|
8
|
0
|
nidogen (entactin)
|
|
12
|
ACTTTAGATGG
|
1
|
23
|
0
|
15
|
0
|
collagen,
type VI, alpha 3
|
|
13
|
GAGTGAGACCC
|
3
|
63
|
0
|
0
|
1
|
Thy-1 cell
surface antigen
|
|
14
|
GTACACACACC
|
0
|
10
|
0
|
0
|
0
|
ESTs / cystatin
S
|
|
15
|
CCACAGGGGAT
|
2
|
38
|
0
|
2
|
1
|
collagen,
type III, alpha 1
|
|
16
|
TTAAAAGTCAC
|
1
|
19
|
1
|
3
|
1
|
TEM6
|
|
17
|
ACAGACTGTTA
|
4
|
74
|
0
|
0
|
0
|
TEM7
|
|
18
|
CCACTGCAACC
|
1
|
18
|
0
|
1
|
0
|
|
|
19
|
CTATAGGAGAC
|
1
|
18
|
1
|
1
|
0
|
TEM8
|
|
20
|
GTTCCACAGAA
|
0
|
9
|
0
|
3
|
0
|
collagen,
type I, alpha 2, transcript B†
|
|
21
|
TACCACCTCCC
|
0
|
9
|
4
|
1
|
1
|
ESTs / pregnancy
specific beta-1-glycoprotein 1
|
|
22
|
GCCCTTTCTCT
|
1
|
17
|
3
|
1
|
2
|
TEM9 (endo180
lectin)
|
|
23
|
TTAAATAGCAC
|
2
|
33
|
0
|
4
|
0
|
collagen,
type I, alpha 1
|
|
24
|
AGACATACTGA
|
1
|
16
|
1
|
0
|
0
|
ESTs, DKFZP434G162
protein
|
|
25
|
TCCCCCAGGAG
|
1
|
16
|
0
|
0
|
0
|
bone morphogenetic
protein 1 (metalloprotease)
|
|
26
|
AGCCCAAAGTG
|
0
|
8
|
0
|
0
|
0
|
|
|
27
|
ACTACCATAAC
|
0
|
8
|
0
|
0
|
0
|
slit (Drosophila)
homolog 3 (MEGF5)
|
|
28
|
TACAAATCGTT
|
0
|
8
|
0
|
0
|
0
|
KIAA0672
gene product
|
|
29
|
TTGGGTGAAAA
|
0
|
8
|
0
|
0
|
0
|
ESTs
|
|
30
|
CATTATCCAAA
|
0
|
8
|
0
|
0
|
0
|
integrin,
alpha 1
|
|
31
|
AGAAACCACGG
|
0
|
8
|
2
|
7
|
0
|
collagen,
type IV, alpha 1
|
|
32
|
ACCAAAACCAC
|
0
|
8
|
0
|
3
|
0
|
|
|
33
|
TGAAATAAAC
|
0
|
8
|
3
|
1
|
1
|
|
|
34
|
TTTGGTTTCC
|
1
|
15
|
0
|
0
|
0
|
ESTs
|
|
35
|
GTGGAGACGGA
|
1
|
15
|
1
|
2
|
1
|
ESTs
|
|
36
|
TTTGTGTTGTA
|
1
|
14
|
2
|
0
|
0
|
collagen,
typeXII, alpha 1
|
|
37
|
TTATGTTTAAT
|
3
|
39
|
0
|
0
|
1
|
lumican
|
|
38
|
TGGAAATGACC
|
15
|
179
|
0
|
40
|
0
|
ESTs / collagen,
type I, alpha 1
|
|
39
|
TGCCACACAGT
|
1
|
13
|
0
|
2
|
0
|
transforming
growth factor, beta 3
|
|
40
|
GATGAGGAGAC
|
3
|
35
|
0
|
18
|
1
|
collagen,
type I, alpha 2, transcript C†
|
|
41
|
ATCAAAGGTTT
|
2
|
23
|
0
|
0
|
0
|
ESTs, DKFZp564O222
mRNA
|
|
42
|
AGTCACATAGT
|
1
|
11
|
2
|
0
|
0
|
ESTs / cell
division cycle 42 (GTP-binding protein)
|
|
43
|
TTCGGTTGGTC
|
4
|
45
|
0
|
19
|
0
|
|
|
44
|
CCCCACACGGG
|
2
|
21
|
0
|
0
|
0
|
ESTs
|
|
45
|
GGCTTGCCTTT
|
1
|
10
|
0
|
10
|
1
|
|
|
46
|
ATCCCTTCCCG
|
1
|
10
|
1
|
0
|
0
|
peanut-like
protein 1
|
|
