Table 1. Previously characterized and novel Pan Endothelial Markers (PEMs).

     The most abundant tags derived by summing the tags from Normal EC (N-EC's) and Tumor EC (T-EC's) SAGE libraries are listed in descending order. N-EC and T-EC SAGE libraries contained 96,694 and 96,588 SAGE tags respectively. For comparison, the corresponding number of SAGE tags found in cultured human umbilical vein endothelial cells (HUVEC), human dermal microvascular endothelial cells (HMVEC), and non-endothelial cell lines (Cell Lines) are shown. The HUVEC SAGE library contained 290,000 tags and the HMVEC library 111,000 tags. Non-endothelial cell lines consisted of 1.8x106 tags derived from a total of 14 different cancer cell lines including colon, breast, lung, and pancreatic cancers, as well as one non-transformed keratinocyte cell line, two kidney epithelial cell lines, and normal monocytes. Tag numbers for each group were normalized to 100,000 transcripts. A 'Description' of the gene product corresponding to each tag is given, followed by alternative names in parenthesis. The sequence CATG precedes all tags and the 15th base (11th shown) was determined as previously described by Velculescu et. al. (Nat Genet 1999 Dec;23(4):387-8).
no. Tag Sequence N-EC's T-EC's HUVEC HMVEC Cell Lines Description
1 CATATCATTAA 247 501 130 87 2 angiomodulin (ANG, IGFBP-7, IGFBP-rP1, Mac25, TAF)
2 TGCACTTCAAG 328 141 0 0 0 hevin
3 TTTGCACCTTT 165 84 191 115 4 connective tissue growth factor (CTGF, IGFBP-rP2)
4 CCCTTGTCCG 131 104 1 1 0 ESTs
5 TTGCTGACTTT 73 131 2 14 1 collagen, type VI, alpha 1
6 ACCATTGGATT 102 67 0 0 2 interferon induced transmembrane protein 1 (9-27, Leu 13)
7 ACACTTCTTTC 104 44 60 62 2 guanine nucleotide binding protein 11
8 TTCTGCTCTTG 71 67 118 72 0 von Willebrand factor
9 TCCCTGGCAGA 66 68 3 13 3 cysteine-rich protein 2 (CRP-2, ESP-1, SmLIM)
10 TAATCCTCAAG 26 106 34 16 1 collagen, type XVIII, alpha 1
11 ATGTCTTTTCT 58 65 17 17 3 insulin-like growth factor-binding protein 4
12 GGGATTAAAGC 40 67 30 14 2 CD146 (S-Endo 1, P1H12, Muc18, MCAM, Mel-CAM)
13 TTAGTGTCGTA 38 69 9 13 0 SPARC (osteonectin, BM-40)
14 TTCTCCCAAAT 20 86 16 64 2 collagen, type IV, alpha 2
15 GTGCTAAGCGG 24 74 0 10 2 collagen, type VI, alpha 2
16 GTTTATGGATA 35 56 11 11 1 matrix Gla protein (MGP)
17 CCCTTTCACAC 52 33 0 0 0 ESTs, Weakly similar to HPBRII-7 protein
18 TGTTCTGGAGA 58 27 18 56 2 gap junction protein, alpha 1, 43kD (connexin 43)
19 AAGATCAAGAT 34 50 2 4 1 actin, alpha 1, skeletal muscle / actin, alpha 2, smooth muscle, aorta
20 TCTCTGAGCAT 32 48 0 0 0 aggrecanase 1 (metalloproteinase with thrombospondin type 1 motifs, 4)
21 CAGGTTTCATA 22 56 0 0 0 small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)
22 GCACAAGTTCT 43 25 6 22 0 calcitonin receptor-like receptor activity modifying protein 2
23 AGCTTGTGGCC 45 23 0 0 0 calcitonin receptor-like receptor activity modifying protein 3
24 CTTCTGGATAA 13 54 12 0 0 cell division cycle 42 (GTP-binding protein, 25kD)
25 CAACAATAATA 42 25 13 6 0 ESTs
26 ACCGGCGCCCG 50 15 0 0 0 tetranectin (plasminogen-binding protein)
27 GGAAGCTAAGT 35 27 0 5 1 osteoblast specific factor 2 (fasciclin I-like)
28 GCAATTTAACC 38 21 0 3 0 solute carrier family 21 (prostaglandin transporter), member 2
29 GATAACTACAT 18 35 4 4 0 angiomodulin (ANG, IGFBP-7, IGFBP-rP1, Mac25, TAF)
30 TATGAGGGTAA 19 30 40 2 0 regulator of G-protein signalling 5
31 CCACGGGATTC 10 39 0 0 0 collagen, type III, alpha 1
32 TTTACAAAGAG 26 21 0 1 1 carboxypeptidase E
33 CCCAGTAAGAT 22 25 0 16 1 cysteine and glycine-rich protein 2 (LIM domain only, smooth muscle)
34 ACAAAGCATTT 26 20 0 14 1 Human insulin-like growth factor binding protein 5 (IGFBP5) mRNA
35 GCCTGTCCCTC 8 38 22 11 0 ESTs / biglycan
36 TACTTTATAAG 25 21 1 1 0 metalloproteinase with thrombospondin type 1 motifs (ADAMTS1, METH-1)
37 TGTTTAATACA 15 29 2 1 1 ESTs / erythrocyte membrane protein band 4.1-like 2
38 GTCCCTGCCTT 18 25 1 1 0 glutathione S-transferase M2 (muscle)
39 GAGCCATCATA 21 21 2 2 1 ESTs / GTP-binding protein overexpressed in skeletal muscle
40 GGCCCTACAGT 26 13 2 3 0 ESTs / KIAA0821 protein
41 GCTAACCCCTG 7 31 0 1 0 ESTs
42 ATCACACAGCT 19 18 0 0 0 thyroid and eye muscle autoantigen D1 (64kD)
43 ACAAGTACTGT 18 19 36 27 0 cadherin 5, VE-cadherin (vascular epithelium)
44 TCACCGTGGAC 20 17 0 1 0 selectin P (granule membrane protein 140kD, antigen CD62)
45 ACATTCCAAGT 18 18 0 1 1 tissue inhibitor of metalloproteinase 3
46 GAGCCTGGATA 6 29 0 0 0 chondroitin sulfate proteoglycan 4 (melanoma-associated)
47 GGCACTCCTGT 22 13 19 12 0 ESTs
48 TCACAGCCCCC 20 15 8 5 0 ESTs
49 TGCCAGGTGCA 10 23 0 1 0 albumin
50 TGGGAAACCTG 11 22 0 1 1 eukaryotic translation initiation factor 4 gamma, 1
51 TTTCATCCACT 20 13 0 2 0 ESTs, KIAA0362 protein
52 AACAGGGGCCA 15 18 0 0 1 ESTs / interferon, alpha-inducible protein (clone IFI-6-16)
53 ACTGAAAGAAG 6 26 0 0 1 complement component 1, s subcomponent
54 ACCGTTCTGTA 8 24 10 6 0 transcription factor 4
55 ATACTATAATT 25 6 2 0 0 ESTs
56 TTTGTATAGAA 17 15 4 5 1 KIAA0393 protein
57 GTAATGACAGA 20 11 1 1 1 stanniocalcin
58 AATAGGGGAAA 13 19 4 1 0 ESTs, KIAA1075 protein
59 GTGCTACTTCT 5 25 2 18 0 collagen, type IV, alpha 1
60 CCGGCCCCTCC 6 24 0 0 1 peanut (Drosophila)-like 2
61 TTGAATTTGTT 19 10 1 1 0 RNA-binding protein gene with multiple splicing
62 CGAGAGTGTGA 22 6 0 0 0 ESTs
63 CCCTGTTCAGC 14 15 38 24 0 tyrosine kinase with IgG and EGF homology domains (Tie)
64 CAGATGGAGGC 18 10 1 9 0 ESTs
65 AGGCTCCTGGC 8 20 0 0 0 ESTs
66 TCTGCTTCTAG 20 8 40 15 0 ESTs
67 GGCTTAGGATG 18 9 10 14 0 ESTs
68 GGTTGTTGCGG 6 21 0 0 1 ESTs
69 ACAAGTACCCA 5 22 4 5 0 P311 protein
70 CTTCTCTTGAG 18 9 1 4 1 basic transcription element binding protein 1
71 GCTAATAATGT 10 17 0 2 0 KIAA1077 protein
72 TGTGCTTTTTT 10 15 1 4 0 KIAA0758 protein / protein kinase, cAMP-dependent, catalytic, alpha
73 CATCACGGATC 17 8 0 1 0 interleukin 1 receptor, type I
74 GCAGCAGCAGC 6 18 0 2 0 T-box 2
75 TGACTGTATTA 13 11 0 0 0 ESTs / amine oxidase, copper containing 3 (vascular adhesion protein 1)
76 GAATGCTCTTG 6 18 0 11 0 gap junction protein, alpha 4, 37kD (connexin 37)
77 GTAGTTCTGGA 18 6 0 5 0 ESTs, clone 23698 mRNA
78 TCCCCTCTCTC 6 17 0 0 0 periodontal ligament fibroblast protein
79 GGGCAGTGGCT 5 18 12 5 0 ESTs, DKFZP586B0621 protein
80 AAATATGTGTT 19 4 13 3 0 ESTs
81 GTCATTTTCTA 11 11 10 2 0 ESTs / transcription factor 8 (represses interleukin 2 expression)
82 CTCTCCAAACC 14 8 0 0 0 complement component 1 inhibitor (angioedema, hereditary)
83 TTAATGTGTAA 4 18 0 0 0 guanylate cyclase 1, soluble, beta 3
84 TCAAGCAATCA 13 9 0 1 0 ESTs
85 GAAGACACTTG 15 7 1 0 0 ESTs
86 GGGTAGGGTGA 6 15 0 0 1 integrin, alpha 7
87 TGGAACAGTGA 10 10 10 5 0 ESTs
88 GAGTGGCTACC 10 9 0 0 0 ESTs
89 GTCAGGGTCCC 13 7 0 9 0 decidual protein induced by progesterone
90 GTCAGTCACTT 14 6 4 1 0 hairy (Drosophila)-homolog
91 AGCAGAGACAA 14 6 1 10 0 natriuretic peptide receptor A - guanylate cyclase A
92 AGCGATGGAGA 9 10 0 0 0 ESTs
93 CGTGGGGTGTA 9 10 17 3 0  
 

 

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