SAGE Project Summary Form

Title: AD-B  

MS Access Files

Date of Library Completion: Feb 2000

Principal SAGE Master: Phillip Buckhaults

Number of Tags: 42,844

Number of Sequence Files: 2,299                Tags/Sequence File: 19

Number of Duplicate DiTags:          427                 Percent: 2%

Number of Linker Tags: 100                 Percent Linker Tags: 0.2%

DiTag Search Compatible (Y or N): Y

Reference:

  Phillip Buckhaults, Carlo Rago, Brad St. Croix, Katharine E. Romans, Saurabh Saha, Lin Zhang, Bert Vogelstein, and Kenneth W. Kinzler (2001)  Cancer Research 61:6996-7001

Detailed Description:

  As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined in gastrointestinal tumors and normal colon tissues.  Normal colonic epithelial cells were isolated from sections of normal colon mucosa from a patient.  Histopathology confirmed that the isolated cells were >90% epithelial.  PolyA RNA was isolated and SAGE was performed.  More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insight into the extent of expression differences underlying malignancy and reveal genes that may prove useful as diagnostic or prognostic markers. (see http://welchlink.welch.jhu.edu /~molgen-g/home.htm).